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cre ert2 coding sequence  (Addgene inc)


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    Structured Review

    Addgene inc cre ert2 coding sequence
    Cre Ert2 Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cre+ert2+coding+sequence/pCAG-ERT2CreERT2+(Plasmid+%2313777)/pm41237766-708-1-9
    Average 93 stars, based on 89 article reviews
    cre ert2 coding sequence - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: Paneth-like transition drives resistance to dual targeting of KRAS and EGFR in colorectal cancer.
    Article Snippet: .. The Cre-ERT2 coding sequence (PCR amplified from the pCAG-ERT2CreERT2, Addgene Cat# 13777) was subsequently inserted downstream of the DEFA5 promoter to generate the DEFA5 promoter-driven Cre-ERT2 plasmid. .. The CMV promoter-driven LoxP-dsRed-STOP-LoxP-eGFP construct was created by inserting the LoxP-dsRed-STOP-LoxP-eGFP DNA fragment (PCR amplified from the pMSCV- LoxP-dsRedSTOP-LoxP-eGFP -Puro-WPRE, Addgene Cat# 32702) into the pCDH-CMV-EF1-blasticidin vector.

    Polymerase Chain Reaction:

    Article Title: Paneth-like transition drives resistance to dual targeting of KRAS and EGFR in colorectal cancer.
    Article Snippet: .. The Cre-ERT2 coding sequence (PCR amplified from the pCAG-ERT2CreERT2, Addgene Cat# 13777) was subsequently inserted downstream of the DEFA5 promoter to generate the DEFA5 promoter-driven Cre-ERT2 plasmid. .. The CMV promoter-driven LoxP-dsRed-STOP-LoxP-eGFP construct was created by inserting the LoxP-dsRed-STOP-LoxP-eGFP DNA fragment (PCR amplified from the pMSCV- LoxP-dsRedSTOP-LoxP-eGFP -Puro-WPRE, Addgene Cat# 32702) into the pCDH-CMV-EF1-blasticidin vector.

    Amplification:

    Article Title: Paneth-like transition drives resistance to dual targeting of KRAS and EGFR in colorectal cancer.
    Article Snippet: .. The Cre-ERT2 coding sequence (PCR amplified from the pCAG-ERT2CreERT2, Addgene Cat# 13777) was subsequently inserted downstream of the DEFA5 promoter to generate the DEFA5 promoter-driven Cre-ERT2 plasmid. .. The CMV promoter-driven LoxP-dsRed-STOP-LoxP-eGFP construct was created by inserting the LoxP-dsRed-STOP-LoxP-eGFP DNA fragment (PCR amplified from the pMSCV- LoxP-dsRedSTOP-LoxP-eGFP -Puro-WPRE, Addgene Cat# 32702) into the pCDH-CMV-EF1-blasticidin vector.

    Plasmid Preparation:

    Article Title: Paneth-like transition drives resistance to dual targeting of KRAS and EGFR in colorectal cancer.
    Article Snippet: .. The Cre-ERT2 coding sequence (PCR amplified from the pCAG-ERT2CreERT2, Addgene Cat# 13777) was subsequently inserted downstream of the DEFA5 promoter to generate the DEFA5 promoter-driven Cre-ERT2 plasmid. .. The CMV promoter-driven LoxP-dsRed-STOP-LoxP-eGFP construct was created by inserting the LoxP-dsRed-STOP-LoxP-eGFP DNA fragment (PCR amplified from the pMSCV- LoxP-dsRedSTOP-LoxP-eGFP -Puro-WPRE, Addgene Cat# 32702) into the pCDH-CMV-EF1-blasticidin vector.

    other:

    Article Title: CD44 + lung cancer stem cell-derived pericyte-like cells cause brain metastases through GPR124-enhanced trans-endothelial migration.
    Article Snippet: In brief Huang et al. show that CD44 CSCderived pericyte-like cells (Cd-pericytes) exhibit remarkable trans-endothelial migration (TEM) potential for effective intravasation and extravasation through GPR124-mediated Wnt7-b-catenin activation, and display potent capacity to de-differentiate into tumorigenic CSCs to initiate brain metastases.. Thus, targeting Cd-pericytes, GPR124, or Wnt7b-catenin signaling significantly inhibits tumor metastasis.



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